Western blot analysis of AHSP expression in Recombinant hAHSP (2 ng) (lane 1), Recombinant mAHSP (2 ng) (lane 2), RBC Lysates Mouse #24 -GFP (lane 3) RBC Lysates Mouse #25 -GFP (lane 4), RBC Lysates Mouse #16 -hAHSP (Lane 5), RBC Lysates Mouse #17 -hAHSP (Lane 6), RBC Lysates Mouse #22 -hAHSP (Lane 7) RBC Lysates Mouse #19 -hAHSP ( Lane 8) and RBC Lysates Mouse #20 -hAHSP( Lane 9). AHSP at 12KD was detected using rabbit anti-AHSP Antigen Affinity purified polyclonal antibody (Catalog # A05948) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Western blot analysis of AHSP expression in Recombinant hAHSP (10ng) (lane 1), Recombinant mAHSP (10ng) (lane 2), mice Spleen cells transfected with TNS9.2-hAHSP (lane 3) mice Spleen cells transfected with TNS9.2 control vector (lane 4), mice Spleen cells transfected with TNS9.2-hAHSP fractionated by MACS using Ter119+ microbeads (Lane 5), mice Spleen cells transfected with TNS9.2 control vector fractionated by Ter119+ (Lane 6), mice Spleen cells transfected with TNS9.2-hAHSP fractionated by Ter119-(Lane 7) and Spleen cells from mice transduced with TNS9.2 control vector fractionated by Ter119-( Lane 8). AHSP at 12KD was detected using rabbit anti-AHSP Antigen Affinity purified polyclonal antibody (Catalog # A05948) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).